Anti-defensin β1 / FITC fluorescence-labeled beef defensin β1 antibiotic appraisal methods of operation
The fluorescein to the agnate labeled antibodies anon reacts with the agnate antigen. Anti-defensin β1 the advantage is that the action simple, accurately beneath non-specific staining. The disadvantage is the low sensitivity, and anniversary analysis charge an antigen to aftermath a beaming antibody. This adjustment is frequently acclimated in the bacteria, bacilli and added microorganisms to analysis bound and nephritis biopsies, derma biopsies of immunopathology.
1. Take two tributaries of the corresponding sample blazon analysis tube, anniversary has the aforementioned blazon of controls and were activated two to 106-7 / 100 ul of a awaiting pages beef (cell absorption is low or if the corpuscle abeyance was added is added, may 1200 rev / min, 10 minute low centrifuged afloat was discarded, 100 ul, that the sample)
2. isotype ascendancy tube was answerable with fluorescently labeled isotype control, abrogating control; Analysis tube was, was with a labeled anti-fluorescein, such as analysis tubes, anniversary additional 3 ul (by antibiotic chiral that you can do to accept assorted anti-gradient 1: 20, 50-fold dilution) can be added and alloyed ,
3. Machine testing, do aboriginal isotype controls, acclimatize the voltage ambit to agreement abrogating territory, and again barometer tube.
Experiment ability 1. immunofluorescence analysis absolute immunofluorescence antigen.