IFABP ELISA Kit Principle
The animal abdominal FABP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent appraisal based on the sandwich assumption with a alive time of 3½ hours. The able architecture of a bowl with twelve disposable 8-well strips allows chargeless best of accumulation admeasurement for the assay. Samples and standards are incubated in microtiter wells coated with antibodies acquainted animal IFABP Biotinylated tracer antibiotic will bind to captured animal IFABP. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will acknowledge with the substrate, tetramethylbenzidine (TMB). The agitator acknowledgment is chock-full by the accession of citric acid. The absorbance at 450 nm is abstinent with a spectrophotometer. A accepted ambit is acquired by acute the absorbance (linear) against the agnate concentrations of the animal IFABP standards (log). The animal IFABP absorption of samples, which are run concurrently.