Measurement of PA production

As PA is rapidly adapted to DAG, it is actual brief in the cell. This agency that it is difficult to admeasurement PA assembly and accordingly to abstraction the role of PA in the cell. However, PLD action can be abstinent by the accession of primary alcohols to the cell. PLD again carries out a transphosphatidylation reaction, instead of hydrolysis, bearing phosphatidyl alcohols in abode of PA. The phosphatidyl alcohols are metabolic dead-ends, and can be readily extracted and measured. Thus PLD action and PA assembly (if not PA itself) can be measured, and, by blocking the accumulation of PA, the captivation of PA in cellular processes can be inferred.